Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDTDLQILSHLQRNGRLTMVELGKLVGLSSPSAAERVRKLEDKGVITGYSANICYEKLNKHVTAFILMEPKSCKH--YAAFATSHPDVAENHRITGMYSYVTKVVTESVHTLEDFIDTSMAHGKPTTLVVLSSSSCHPAF 2ZNZ Chain:C ((27-142)) --EIDKKIIKILQNDGKAPLREISKITGLAESTIHERIRKLRESGVIKKFTAIIDPEALGYSMLAFILVKVKAGKYSEVASNLAKYPEIVEVYETTGDYDMVVKIRTKNSEELNNFLD-----------------------

General information: TITO was launched using: RESULT: Template: 2ZNZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 313 -54061 -172.72 -474.22 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain C : 0.72 3D Compatibility (PKB) : -172.72 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_2ZNZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZNZ-query.scw PDB file : Tito_Scwrl_2ZNZ.pdb: