Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKLTKEELIFLVKELMNPTL----DDEKVSEYLDLLEKNVPYPAPSDLIFWSNE--DYTAEQVVKIALNYKDE 4QKO Chain:A ((6-77)) -SEYTEKEFLEFVKDIYTNNKKKFPTEESHIQAVLEFKKLTEHPSGSDLLYYPNENREDSPAGVVKEVKEWRA-

General information: TITO was launched using: RESULT: Template: 4QKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 185 -31034 -167.75 -470.20 target 2D structure prediction score : 0.86 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -167.75 2D Compatibility (Sec. Struct. Predict.) : 0.86 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.725

(partial model without unconserved sides chains): PDB file : Tito_4QKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QKO-query.scw PDB file : Tito_Scwrl_4QKO.pdb: