Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVNFYDVAYDLENA---LRGSEEFTRLKNLYDEVNADESAKRMFENFRDVQLRLQQKQMAG-EEITQEEVTQAQKTVALVQQHEKISQLMEAE-QRMSMLIGELNKIIMKPLEELYGSVEG- 2PIH Chain:A ((2-124)) TLYSKKDIVQQARNLAKMISETEEVDFFKRAEAQINENDKVSTIVNQIKALQKQAVNLKHYEKHEALKQVEAKIDALQEELEEIPVIQEFRDSQMEVNDLLQLVAHTISNQVTNEIITSTGGD

General information: TITO was launched using: RESULT: Template: 2PIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 217 -11862 -54.66 -101.38 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -54.66 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_2PIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PIH-query.scw PDB file : Tito_Scwrl_2PIH.pdb: