Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLSQWQPSRKSDVPLHRQIEQYMKDKILHGEWAVGTKIPSQRTLADMFQVNRSTVTAAIDELTSQGLLEGRKGGGTKVVNSTWSVLTAEPPLDWSDYVRSGIHRANSSIIQAINQNEPRADIIRLGTGELSPDLVPADTIGRMFQQI---NPGVLSLGYEQPKGNRQLREAVADHLKG-KKIHVSPSAILIVSGALQALQLISIGLLKRDSVILTEKPSYLQSLHVFQSAGMRLRGLPMDDEGVKAGLVSSNRKQYGGQ-----LLYTIPSFHNPTGTVMSEQRRKEIISLSKKEQMPIIEDDAYGDLWFEEKPPQPLKAMDHEGNILYLGAFSKTVSPGLRIGWLAGPEPVIERLADIKMQTDYGSSGLSQWAAAEWLSQGYYEE-HLTWVRRKLKERRDAAVHFLERYAGDIATWRIPAGGFYIWVTFHKNLPVSRFFYELLKRQ-VLVNPG--YIYDGEDRNSIRLSYSYASLGDLETGIKAAAETARRLMMS 2ZC0 Chain:D ((36-399)) --------------------------------------------------------------------------------------------------------------------------LISLAAGDPDPELIPRAVLGEIAKEVLEKEPK--SVMYTPANGIPELREELAAFLKKYDHLEVSPENIVITIGGTGALDLLGRVLIDPGDVVITENPSYINTLLAFEQLGAKIEGVPVDNDGMRVDLLEEKIKELKAKGQKVKLIYTIPTGQNPMGVTMSMERRKALLEIASKYDLLIIEDTAYNFMRYEGGDIVPLKALDNEGRVIVAGTLSKVLGTGFRIGWIIAEGEILKKVLMQKQPIDFCAPAISQYIALEYLKRGYFEKYHLEGALLGYKEKRDIMLKALENHLPN-AEFTKPIAGMFVMFFLPEGADGISFANELMEREGVVVVPGKPFYTDESGKNAIRLNFSRPSKEEIPIGIKKLAK--------

General information: TITO was launched using: RESULT: Template: 2ZC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2013 -203111 -100.90 -578.66 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : -100.90 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_2ZC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZC0-query.scw PDB file : Tito_Scwrl_2ZC0.pdb: