Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYRQMLIHRCDIYHEAAQAPSAGRFGIPADRLQPVISYPDTPDEQDVPCYFTEKTQQLIQEEPDQTVYHSFLVHFPLSADIRVNDKIIW-----ENHKYILKLPK-R-IRHHHWEVVAVRDESL 2KZ4 Chain:A ((4-110)) ----GRLRDRITIQTLKQTR---DITGEILE------T--WED-GHTLWASVNMVSSKEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRLEILCREGA--

General information: TITO was launched using: RESULT: Template: 2KZ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 413 -52367 -126.80 -523.67 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -126.80 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.273

(partial model without unconserved sides chains): PDB file : Tito_2KZ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KZ4-query.scw PDB file : Tito_Scwrl_2KZ4.pdb: