Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAFIFLSAYVKKLAGSELETDTREIL--EENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASLCHYSTQADKDRLTEHMD--DPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVLPIEEEGEG 2Y8P Chain:A ((19-208)) ------------KHDYTNPPWNAKVPVQRAMQWMPISQKAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKAS-TSGRDVYRRMGWSGEPTTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSS---------------DRKKAISKINDLDADEFLEHVARN------------HPAP-QAPRYIYKLEQALDAMLEHHH---------------------

General information: TITO was launched using: RESULT: Template: 2Y8P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 902 -65614 -72.74 -352.76 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -72.74 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_2Y8P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y8P-query.scw PDB file : Tito_Scwrl_2Y8P.pdb: