Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGEIRLIPYVTNEQIMDVNELPEGIKVIKAPEMWAKGVKGKNIKVAVLDTGCDTSHPDLKNQIIGGKNFTDDDGGKEDAISDYNGHGTHVAGTIAANDSNGGIAGVAPEASLLIVKVLGGENGSGQYEWIINGINYAVE--QKVDIISMSLGGPSDVPELKEAVKNAVKNGVLVVCAAGNEGDGDERTEELSYPAAYNEVIAVGSVSVARELSEFSNANKEIDLVAPGENILSTLPNKKYGKLTGTSMAAPHVSGALALIKSYEEESFQRKLSESEVFAQLIRRTLPLDIAKTLAGNGFLYLTAPDELAEKAEQSHLLTL 2WV7 Chain:F ((3-305)) ---KFRLIPYKQVDKVSALSEVPMGVEIVEAPAVWRASAKGAGQIIGVIDTGCQVDHPDLAERIIGGVNLTTDYGGDETNFSDNNGHGTHVAGTVAAAETGSGVVGVAPKADLFIIKALSGD-GSGEMGWIAKAIRYAVDWR-QMRIITMSLGGPTDSEELHDAVKYAVSNNVSVVCAAGN--------NEFAYPAAYNEVIAVGAVDFDLRL--------EIDIVAPGVGIKSTYLDSGYAELSGTAMAAPHVAGALALIINLAEDAFKRSLSETEIYAQLVRRATPIGFTAQAEGNGFLTL------------------

General information: TITO was launched using: RESULT: Template: 2WV7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1956 -203455 -104.02 -726.62 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain F : 0.85 3D Compatibility (PKB) : -104.02 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_2WV7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WV7-query.scw PDB file : Tito_Scwrl_2WV7.pdb: