Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANFIEKITYLGTPAIKAGN--EHLEMIVVPEWGSNVISLVDKTTNVQLLREPETAESFH-DTPTLYGIPILFP-PNRISDGTFSFRGRTYHFDINEKDKHNHLHGFLYHEKWNVVTTKQTDEGVIVETEIDLSELPHVQKQFPHHAVVRMTYTIKENTLFKHATVMNKGKEAFPWGIGYHTTFIFPA-ESSLFSLTADQQWELDERLLPTGKLMDVPYKEALHEGMDLRHKQLDDVFLSSYQKRGGENQAVIYHQHAHISIIYKADEQFKHWVVYNADGKQGYLCPEPYTWVTNAVNLDLPSSLTGLQVLEPGEETTAKSSITIELNHQ 3K25 Chain:A ((12-288)) -------------LTYHLEDDQSLARLSLVPGRGGLVTEWTVQG--QPILYFDRER-FQDPSLSVRGGIPILFPICGNLPQDQFNHAGKSYRLK---------QHGFARDLPWEVIGQQTQ-DNARLDLRLSHND--ATLEAFPFAFELVFSYQLQGHSLRIEQRIANLGDQRMPFSLGFHPYFFCREKLGITLAIPANDYLDQK-----TGDCHGYD------GQLNLTSPELDLAFTQ----IS-QPRAHFIDPDRNLKIEVSFSELYQTLVLWTVAG-KDYLCLEPWSGPRNALNSG-----EQLAWVEPYSSRSAWVNFQVST---

General information: TITO was launched using: RESULT: Template: 3K25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1529 22274 14.57 81.89 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 14.57 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_3K25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K25-query.scw PDB file : Tito_Scwrl_3K25.pdb: