Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYASKLKKGDEIRIVSPATSMSILSN--EAKIQAKTAL-ERLGYRVTIAEHANECNEFDSSSIESRVHDLHAAFFDPGVKAILTTLGGFNSNQLLRYLDYEKIKRHPKILCGYSDITALCNAIYQKTGLVTYSGPHFSTFAMKKG--LDYTEEYFLSCCASDDP-FEIHPSSEWSDD--RWFLDQ--ENRRFYPNNGPVVIQ-EGYAEGTLIGGNLCTLNLLQGTEYFPE---TEHTILLIEDDYMSD-IHMFDRDLQSLIHLPAFSHVKAILIGRFQKASNV--SIDLVKAMIETKKELSGIPIIANINAGHTSPIATFPIGGTCRIEAISGTSRIWIDKH 5F5X Chain:A ((6-342)) IKPKRLQAGDIVATVSPSWGGAGDSEIRWRYEQGVKRLEEVFGLTVVPMPNSLKGSEFIYNNPQARAEDLMTAFQDTRVKAIIANIGGQDSIRLLPYIDFNAIRENPKIFMGYADVTISHLFCH-KAGLSSFYGPAILTDFAENVEMDPYTVEMVNRTLFSNEMIGEIQPAPEWTSERLEWIEINKDTRRTMQQNNGYELLQGSTTVQGRLIGGCIEVLEFAKGTELWPEKKHWEDSILFFETSEDHPEPSYIKYWLRNYAAQGILQKAKGIIFGKPKDEMYYEEYKHEILQVMKEH-NLEDLPILYNLNFGHTEPKFILPYGSMAEIDCENGSFSILE---

General information: TITO was launched using: RESULT: Template: 5F5X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1829 -15590 -8.52 -48.72 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -8.52 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_5F5X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F5X-query.scw PDB file : Tito_Scwrl_5F5X.pdb: