Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------MNTAYRVWDGEQMHYWDDEGLSLIIKSNGDWTLKRL------YTDVLVPVVDSTNRNAALMWGAKVRGKFIYDRSIVKITSDDKESSDVCEVKFSDGVFQVDVSK-------ISADYDVTAVGWVEYATIEVIGDVYQNPE---LLEGVK- 4O6G Chain:A ((1-174)) SVTIGVDLSTDLQDWIRLSGMNMIQGSETNDGRTILWNKGGEVRYFIDRLAGWYVITSSDRMSREGYEFAAASMSVIEKYLYGYFGGSVRSERELPAIRAPFQPEELMPEYSIGTMTFAGRQRDTLIDSSGTVVAITAADRLVELSHYLDVSVNVIKDSFLDSEGKPLFTLWKD

General information: TITO was launched using: RESULT: Template: 4O6G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 490 10960 22.37 81.79 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 22.37 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.123

(partial model without unconserved sides chains): PDB file : Tito_4O6G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O6G-query.scw PDB file : Tito_Scwrl_4O6G.pdb: