Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDYSQFPRKNILCVDMKSFYASVSAVTMGLNPLTCYLAVVGNTD--RQGSVVLAASPALKKDFGIKTGSRLFEIPEDP-RIHIVNPQMKLFIRVSTEITKLFYRFVPEKCVHTYSIDESFLDAGK-----EDPEEMAKAIQSSMWREFGLMCTVGIGDNMLLSKLALDLESKKTKSGIARWRYEDVPNKLWKVRPLSKMWGIGGRMERNLNRMGISTIGQLAKFPLELLEKKFGI-MGNQLYYHAHGIDLSEIGAPLMQGQISFGKSQILLRDYTRSEEIKAVLLEICEEVARRARTHNKVGRTISLGIGYSKDELGGGFHRSKTIDLPTSITMDIYRCCLMLFNKFY--SGKTVRSVSVTLSNIEDDVNQQLSLFEVDNEKRRKLGFVMDGIRSKYGSKAILRAVSYTPAGTALQRAGLTGGHKS 4TQR Chain:A ((2-342)) ----------IVLFVDFDYFYAQVEEVLN-PSLKGKPVVVCVFSGRFEDSGAVATANYEARK-FGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSE--KIEIASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAADMAKP---NGIKVIDDEEVKRLIRE-LDIADVPGIGNITAEKLKKLGINKLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRT---RVRKSIGRIVTMKRNSRNLEEIKPYLFRAIEESYYKLDKRI--PKAIHVVAVTEDL---DIVSRGRTFPHGISK-ETAYSESVKLLQKILEEDERKIRRIGVRFSKFIE-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TQR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1498 24759 16.53 75.03 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 16.53 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_4TQR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TQR-query.scw PDB file : Tito_Scwrl_4TQR.pdb: