Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEALKRKIEEEGVVLSDQVLKVDSFLNHQIDPLLMQRIGDEFASRFAKDGITKIVTIESSGIAPAVMTGLKLGVPVVFARKHKSLTLTDNLLTASVYSFTKQTESQIAVSGTHLSDQDHVLIIDDFLANGQAAHGLVSIVKQAGASIAGIGIVIEKSFQPGRDELVKLGYRVESLARIQSLEEGKVSFVQEVHS 4M0K Chain:A ((29-199)) -ETLKQAIRTVPDFPEPGIQFKD-ITPVLGHPELLRLAIEALLEPFQEQEITKVVGIESRGFILGGMLAHHLDAGFVPVRKKGKLP--YQTLAESYQLEYG--TDTIEMHIDAIEPGDRVLIHDDVIATGGTAEATIRLVERAGGEVVGCAFLIELTGLQGRKRLP-AHVPVHTVLQL----------------

General information: TITO was launched using: RESULT: Template: 4M0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 890 -132957 -149.39 -777.52 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -149.39 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_4M0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M0K-query.scw PDB file : Tito_Scwrl_4M0K.pdb: