Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLLMLTTGGTIASVEGENGLAP-GVKADELLSYVSKLDN----DYTMETQSLMNIDSTNMQPEYWVEIAEAVKENYDAYDGFVITHGTDTMAYTSAALSYMLQHAKKPIVITGSQIPITFQKTDAKKNITDAIRFACE-GVGGVYVVFDGRVIQGTRAIKLRTKSYDAFESINYPYIAFINEDGIEYNKQVTEPE---NDTFTVDTSLCTDVCLLKLHPGLKPEMF-DALKSMYKGIVIESYGSGGVPFEGRDILSKVNELIESGIVVVITTQCLEEGEDMSIYEVGRRVNQDLIIRSRNMNTEAIVPKLMWALGQSSDLPVVKRIMETPIADDVVL 2P2D Chain:C ((24-346)) -KSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPL-MDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPLL---EAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILRSYGVGNAP-QNKAFLQELQEASDRGIVVVNLTQCMSGKVNMG-----NALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAM----------

General information: TITO was launched using: RESULT: Template: 2P2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1728 29080 16.83 94.42 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : 16.83 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.557

(partial model without unconserved sides chains): PDB file : Tito_2P2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P2D-query.scw PDB file : Tito_Scwrl_2P2D.pdb: