Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKKTWKRFLHFSSAALAAGLIFTSAAPAEAAFWGASNELLHDPTMIKEGSSWYALGTGLTEERGLRVLKSSDAKNWTVQKSIFTTPLSWWSNYVPNYGQNQWAPDIQYYNGKYWLYYSVSSFGSNTSAIGLASSTSISSGG----WKDEGLVIRSTSSNN-YNAIDPELTFDKDGNPWLAFGSFWSGIKLTKLDKSTMKPT--GSLYSIAARPNN---------GGALEAPTLTYQNGYYYLMVSFDKCCDGVNSTYKIAYGRSKSITGPYLDKSGKSMLEGGGTILDSGNDQWKGPGGQDIVN---GNILVRHAYDANDNGIPKLLINDLNWS-SGWPSY 1GYH Chain:B ((2-303)) ------------------------------------AKQVDVHDPVMTREGDTWYLFSTG----PGITIYSSKDRVNWRYSDRAFATEPTWAKRVSPSFDGHLWAPDIYQHKGLFYLYYSVSAFGKNTSAIGVTVNKTLNPASPDYRWEDKGIVIESVPQRDLWNAIAPAIIADDHGQVWMSFGSFWGGLKLFKLNDDLTRPAEPQEWHSIAKLERSVLMDDSQAGSAQIEAPFILRKGDYYYLFASWGLCCRKGDSTYHLVVGRSKQVTGPYLDKTGRDMNQGGGSLLIKGNKRWVGLGHNSAYTWDGKDYLVLHAYEAADNYLQKLKILNLHWDGEGWPQ-

General information: TITO was launched using: RESULT: Template: 1GYH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1602 205 0.13 0.73 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 0.13 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_1GYH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GYH-query.scw PDB file : Tito_Scwrl_1GYH.pdb: