Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMQEETF--YSVRMRASMNGSHEDGGKHISGGERLIPFHEMKHTVN--ALLEKG--------LSHSRGKPDFMQIQFEEVHES---------IKTIQPLPVHTNEVSCPEEGQKLARLLLEKEGVSRDVIEKAYEQIPEW---SDVRGAVLFDIHTGKRMDQTK----EKGVRVSRMDWPDANFEKWALHSHVPAHS-----------------------RIKEALALASKVSRHPAV--------VAELC--------WSDDPDYIT----GYVA-----------------GKKMGYQR--------ITAMK-EYGTEEGCRVFFIDGSNDVNTYIHDLEKQPILIEWEEDHDS--------------------------- 2WZP Chain:R ((1-375)) MLEANVYDNFNPNYYNISDFSMPNGKKE----KRGLPIPKARCQVINYELWETGYLYTSSATLTVSVEVGDIVQILFPEVVPIEEALGKKKKLNLDMVYLVTDVDESNKATLKNYFWAMIESLDVPNAITKTTNFAIIDYLIDPNKNNLMSYGYFFNSSIFAGKATINRKAETSSAHDVAKRIFSKVQFQPTTTIQHAPSETDPRNLLFINFASRNWNRKRITTRVDIKQSVTMDTETIVERSAYNFAVVFVKNKATDDYTDPPKMYIAKNNGDVIDYSTYHGDGTDLPDVRTAKTLFYDRDDHGNPPELSTIKVEISPSTIVTRLIFNQNELLPLYVNDL----VDIWYEGKLYSGYIADRVKTEFNDRLIFVESGDKPNVI

General information: TITO was launched using: RESULT: Template: 2WZP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain R - contact count / total energy / energy per contact / energy per residue : 1121 22270 19.87 88.73 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain R : 0.67 3D Compatibility (PKB) : 19.87 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.040

(partial model without unconserved sides chains): PDB file : Tito_2WZP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WZP-query.scw PDB file : Tito_Scwrl_2WZP.pdb: