Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKKAIIPAGGFGTRNLPVTKVIPKEMFPVGSKPVIHYLVEELKESGIEDILMVVSSHKNLIVDYFDSSLALEAFLASKNK-LHLLREHPIPDIRIHYVRQPYAKGLGDAISFGKQFAGGEPFAVVLPDDLIFSANQP-ALGQLIEAYTKYQSSVIGLKETKTEDLHHYGVIKGEPVEKGLYRIQDIVEKPK-QNPPSHFAAAGRYIFTPDIFNELEALEADSGGEVQVTDAIKASLGACTVYGKLLEGERYDIGLQKDYLKLIYDMLKTEKNPQ 2UX8 Chain:F ((14-272)) -LRKAVFPVAGLGTRFLPATKAMPKEMLPVVDRPLIQYAVDEAVEAGIEQMIFVTGRGKSALEDHFDIAYELEATMAARGKSLDVLDGTRLKPGNIAYVRQQEPMGLGHAVWCARDIVGDEPFAVLLPDDFMF--GQPGCLKQMVDAYNKVGGNLICAEEVPDDQTHRYGIITPGTQDGVLTEVKGLVEKPAPGTAPSNLSVIGRYILQPEVMRILENQGK------QLTDAMQRMIGDQPFHGVTFQGTRYDCGDKAGFIQ-------------

General information: TITO was launched using: RESULT: Template: 2UX8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1266 -26138 -20.65 -104.55 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain F : 0.80 3D Compatibility (PKB) : -20.65 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_2UX8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2UX8-query.scw PDB file : Tito_Scwrl_2UX8.pdb: