Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLWELFVAAILGIVEG--LTEYAPVSSTGHMIIVDDI-WLKSSNLMSEEAANSFKVVIQLG---SILAVAIVFK-DRILNLLG-LKKNITSDQEQGHKLSI---AQIAVGLVPAAVLGFLFED-Y-IDEYL--------FSVKTVAIGLIAGAILMLFADWVNKRKTATDTL-DRISYKQAIAVGL------------------FQCLSL-WPGFSRSGSTISGGVILGL-NHRAAADFTFIMAMPIMMGASFLSLVKH-WDSLSSDLMPFFIVGFICAFVVALFVVRFFLRLINKIKLVPFAIYRIILGVILLLIMM 3ZX7 Chain:A ((5-300)) AEGYEQIEVDVVAVWKEGYVYENRGSTSVDQKITITKGMKNVNSETRTVTATHSIGSTISTGDAFEIGSVEVSYSHSHEESQVSMTETEVYESKVIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTQSIPQVITSRAKIIVGRQIILGKTEIRIKHAERKEYMTVVSRKSWPAATLGHSKLFKFVLYEDWGGFRIKTLNTMYSGYEYAYSSDQGGIYFDQGTDNPKQRWAINKSLPLRHG----DVVTFMNKYFTRSGLCYDDGPATNVYCLDKREDKWILEVVGLVPR-------------------

General information: TITO was launched using: RESULT: Template: 3ZX7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1144 -5721 -5.00 -22.61 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -5.00 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.074

(partial model without unconserved sides chains): PDB file : Tito_3ZX7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZX7-query.scw PDB file : Tito_Scwrl_3ZX7.pdb: