Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTENAKTNQTLVENSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERLLAIGGQPVATVKEYTEHASITDGGNETSASEMVQALVNDYKQISSESKFVIGLAEENQDNATADLFVGLIEEVEKQVWMLSSYLG 1JIG Chain:D ((1-145)) -------STKTNVVEVLNKQVANWNVLYVKLHNYHWYVTGPHFFTLHEKFEEFYNEAGTYIDELAERILALEGKPLATMKEYLATSSVNEGTSKESAEEMVQTLVNDYSALIQELKEGMEVAGEAGDATSADMLLAIHTTLEQHVWMLSAFL-

General information: TITO was launched using: RESULT: Template: 1JIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 601 -61822 -102.86 -426.36 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain D : 0.86 3D Compatibility (PKB) : -102.86 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.579

(partial model without unconserved sides chains): PDB file : Tito_1JIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JIG-query.scw PDB file : Tito_Scwrl_1JIG.pdb: