Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLISIDSRKHLFYEEYGQGIPIIFIHPPGMGRKVFYYQRL-LSKHFRVIFPDLSGHGDSDHIDQPASISYYANEIAQFMDALHID-KAVLFGYSAGGLIAQHIGFTRPDKVSHLILSGAYPAVHNVIGQKLHKLGMYLLEKNPGLLMKILAGSHTKDRQLRSILTDHMKKADQAHWHQYYLDSLGYNCIEQLPRLEMPMLFMYGGLRDWTFTNAGYYRRSCRHAEFFRLEYQGHQLPTKQWKTCNELVTGFVLTHHS 4UHF Chain:A ((17-124)) ----------ELAFEDTGTGLPVLLVHGFPLDRTMWKAQREELCDEFRVIVPDLRGFGESQVIPGVATMEAMADDLAGLCNHLGLTGKIVLGGLSMGGYVAFAFARKYRDRLAGLILC--------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4UHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 526 -34306 -65.22 -323.64 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -65.22 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_4UHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UHF-query.scw PDB file : Tito_Scwrl_4UHF.pdb: