Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MLVNSKEIVMKELLDRY---------MDQLHMACTCQVCQNDVLALSLNKVSP-SYVTDFKKIAYTK-----AELVDKQKNTAMLVILAESAAVVSE----SPSDLCQTKQEEAFIN-- 3DT5 Chain:A ((15-133)) RLKAIEDRLEKFYIPLIKAFSSYVYTAQTEDEIETIITCR---RYLAGNNLLRVLPMHFKFKADKIAGSANWTFYAKEDFEQWKEALDVLWEEFLEVLKEYYTLSGTEISLPEKPDWLIGYK

General information: TITO was launched using: RESULT: Template: 3DT5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 291 20527 70.54 216.07 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 70.54 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_3DT5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DT5-query.scw PDB file : Tito_Scwrl_3DT5.pdb: