Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDIHCHILPAMDDGAGDSADSIEMARAAVRQGIRTIIATPHHNNGVYKNEPAAVREAADQLNKRLIKEDIPLHVLPGQEIRIYGEV--EQDLAKRQLLSLNDTKYILIEFPFD-----HVPRYAEQLFYDLQLKGYIPVIAHPERNREIRENPSLLYHLVEKGAASQITSGSLAGIFGKQLKAFSLRLVEANLIHFVASDAHNVKTRNFHTQEALYVLEKEFGSELPYMLTENAELLLRNQTIFRQPPQPVKRRKLFGFF 1M65 Chain:A ((3-229)) PVDLHMHTVAS-THAYSTLSD---YIAQAKQKGIKLFAITDHGPDMEDA---PHHWHFIN-MR-IWPRVVDGVGILRGIEANIKNVDGEIDCSGK----MFDSLDLIIAGFHEPVFAPHDKATNTQAMI-ATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNSS-------NCREVAAAVRDAGGWVALGSDSHTAFTMG-EFEECLKILD-AVDFPPERILNVSPRRLLN---------------------

General information: TITO was launched using: RESULT: Template: 1M65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1236 -19077 -15.43 -90.84 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -15.43 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_1M65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M65-query.scw PDB file : Tito_Scwrl_1M65.pdb: