Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVAIASDHGGVHIRNEIKELMDELQIEYIDMGCDCGSG-SVDYPDYAFPVAEKVVSGEVDRGILICGTGIGMSISANKVKGIRCALAHDTFSAKATREHNDTNILAMGERVIGPGLAREIAKIWLTTEFTG-GRHQTRIGKISDYEEKNL 3K8C Chain:B ((24-169)) -RVAIGTDHPAFAIHENLILYVKEAGDEFVPVYCGPKTAESVDYPDFASRVAEMVARKEVEFGVLACGSGIGMSIAANKVPGVRAALCHDHYTAAMSRIHNDANIVCVGERTTGVEVIREIIITFLQTPFSGEERHVRRIEKIRAIE----

General information: TITO was launched using: RESULT: Template: 3K8C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 789 -45087 -57.14 -313.10 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -57.14 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_3K8C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K8C-query.scw PDB file : Tito_Scwrl_3K8C.pdb: