Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKVSVIMTSYNKSDYVAKSISSILSQTFSD---FELFIMDDNSNE--ETLNVIRPFL-NDNRVRFYQSDISGVKERTEKTRYAALINQAIEMA----EGEYITYATDDNIYMPDRLLKMVRELDTHPEKAVIYSASKTYHLNENRDI------VK--ETVR---PAA-------------Q-----VTWN--APCAIDHCSVMHRYSVLEKVKEKFGSYWDESPAFYRIGDARFFWRVNHFYP-FYPLDEELDLNYITDQSIHFQLFELEKNEFVRNLPPQRNCRELRESLKKLGMG 4FIX Chain:A ((180-420)) -ANIAVGIPTFNRPADCVNALRELTADPLVDQVIGAVIVPDQGERKVRDHPDFPAAAARLGSRLSIHDQPNLG-------G-SGG-YSRVMYEALKNTDCQQILFMDDDIRLEPDSILRVLAMHRFAKAPMLVGGQM--LNLQEPSHLHIMGEVVDRSIFMWTAAPHAEYDHDFAEYPLNDNNSRSKLLHRRIDVDYNGWWTCMIPRQVAEEL----GQPL---PLFIKWDDADYGLRAAEHGYPTVTLPGAAIWHMAWS--------------------------------------

General information: TITO was launched using: RESULT: Template: 4FIX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 927 -6640 -7.16 -33.37 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -7.16 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_4FIX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FIX-query.scw PDB file : Tito_Scwrl_4FIX.pdb: