Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMTNNTVLITGGSAGIGLELAKRLLELGNEVIICGRSEARLAEA----KQQLPN--IHTKQCDVADRSQREALYEWALKEYPNLNVLVNNAGIQKEIDFKKGTEELFVDGDEIELNFQAPVHLSALFTPHLMKQPEAAIVQVTSGLAFNPLAVYPVYCATKAALHSFSLTLRHQLRDTSVEVIEMAPPMVDTGLNQKSRDKQGLTYRGISSEEYVQYFLDGLKEGKQEITNERVEGLRDATRADYDRLFEQMNTQEN 3LF2 Chain:D ((6-162)) --LSEAVAVVTGGSSGIGLATVELLLEAGAAVAFCARDGERLRAAESALRQRFPGARLFASVCDVLDALQVRAFAEACERTLGCASILVNNAGQGRVSTFAETTDEAW--SEELQLKFFSVIHPVRAFLPQLESRADAAIVCVNSLLASQP---EPHMVATSAA----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3LF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 677 -25506 -37.67 -168.91 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.69 3D Compatibility (PKB) : -37.67 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.440

(partial model without unconserved sides chains): PDB file : Tito_3LF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LF2-query.scw PDB file : Tito_Scwrl_3LF2.pdb: