Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQTIRVELTSTKKPKPDPNQ-LSFGRVFTDHMFVMDYAADKGWYDPRIIPYQPLSMDPAAMVYHYGQTVFEGLKAYVSEDDHVLLFRPEKNMERLNQSNDRLCIPQIDEEQVLEGLKQLVAIDKDWIPNAEGTSLYIRPFIIATEPFLGVAASHTYKLLIILSPVGSYYKEG-IKPVKIAVESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLWLDGIEKKYIEEVGSMNIFFKING-----EIVTPMLNGSILEGITRNSVIALLKHWG-LQVSERKIAIDEVIQAHKDGILEEAFGTGTAAVISPVGELIWQDETLSINNGETG-EIAKKLYDTITGIQKGAVADEFGWTTEVAALTESK 2HG8 Chain:B ((10-361)) -----LQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWN-DKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPRRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQ-LTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTAAQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEW-------------

General information: TITO was launched using: RESULT: Template: 2HG8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1804 -38819 -21.52 -113.17 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -21.52 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_2HG8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HG8-query.scw PDB file : Tito_Scwrl_2HG8.pdb: