Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKVNQILEEKVMPIAGRIAGQRHLQALRDGIILTMPLIIIGSFFLIIGNLPIPGYAEFMAKTFGSSWS-----EKLAYPVDATFEIMGLVAAFGIAYRLAEKYGVDALSAGAISLAAFLLATPYQVPFMPDGATKEIMVGGGIPLSLMGSKGLFVAMIIAMVSTEIYRLIIQRNLVFKMPDGVPPAVSKSFVALIPGFAVIFLIWAARLIVEATPFESLHNIVSVLLGTPLSILGGSLGGSLVAEAVKMLLWACGLHGANIVGGVMAPIWYGAMDANRI-AFQAGEELPKIFTQQFFDIWVNIGGSGATLALVVTMFLRARSKQMKQLGKLAVG--PAIFNINEPIIFGMPIVMNPMLLLPFIITPLVTVTLTYIGMSTGLVAKPAGIAVPWTMPPIFSGYLATGGKVSGAVMQAINIAVSFVVYYPFFRMWDKQKLKEENDLELVQTPAATDDKEAAL-- 4WZI Chain:A ((85-576)) VTPFAQVLASLRSVRNNFTIQKLAMETLEELDWALDQLETIQT-YRSVSEMASNKFKRMLNRELTHLSEMSRCGNQVSEYISNTFLDKENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLTAIMYAI-FQERDLLKTFRI----------SSDTFITYMMTLED--------------HYHSDVAY--HNSLHAADVAQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDV-----DHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHADIFMNLTKKQRQTLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHAADL-SNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEI------SPMADKHTACVEKSQVGFIDYI--------VHPLWETW--ADLVQPDAQDILDTLEDNRNWYQAMIP

General information: TITO was launched using: RESULT: Template: 4WZI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1635 -60147 -36.79 -149.62 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -36.79 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.185

(partial model without unconserved sides chains): PDB file : Tito_4WZI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WZI-query.scw PDB file : Tito_Scwrl_4WZI.pdb: