Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------MADNNKMSREEAGRKGGETTSKNHDKEFYQEIGQKGGEATSKNHDKEFYQEIGEKGGEA----TSKN------------HDKEFYQEIGEKGGEATSENHDKEFYQEIGRKGGEATSKNH--------DKEFYQEIGSKGGNARNND- 1GZS Chain:B ((1-165)) GSLTNKVVKDFMLQTLNDIDIRGSASKDPAYASQTREAILSAVYSKNKDQCCNLLISKG------INIAPFLQEIGEAAKNAGLPGTTKNDVFTPSGAGANPFITPLISSANSKYPRMFINQHQQASFKIYAEKIIMTEVAPLFNECAMPTPQQFQLILENIANKYIQNTP

General information: TITO was launched using: RESULT: Template: 1GZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 286 -12966 -45.34 -110.82 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -45.34 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_1GZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GZS-query.scw PDB file : Tito_Scwrl_1GZS.pdb: