Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIRTENFQTFIRLYPVVTFILALQAVLWLFFSLPAHSVVLWRDTVTGYNLGVANGEWWRLITPILLHAGFTHLLFNSMSIFLFAPALERMLGKARFLLVYAGSGIIGNIGTYVTEPLDYVHVGASGAIFGLFGVYLFMVLFRNELI-GQ--EHSKMIITLLAFAVLMSFINSNINMMAHLFGLCGGFLLSFLCVQKKERRY 2XOV Chain:A ((2-180)) ------------RAGPVTWVMMIACVVVFIAMQILGD--QEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFS--GPWFGGLSGVVYALMGYVWLRGERDPQSGIYLQRGLIIFALIWI-VAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSL------

General information: TITO was launched using: RESULT: Template: 2XOV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 837 -151608 -181.13 -861.41 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -181.13 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.239

(partial model without unconserved sides chains): PDB file : Tito_2XOV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XOV-query.scw PDB file : Tito_Scwrl_2XOV.pdb: