Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTPKKHKKFKAKMILQIIMVIIGGIIAAYGLETVLIPNSVSDGGVTGLSIVGSQLFNLPLGILIAVINIPFVWLGYKQIGKSFALLSIIGIVSLAAGTSFFHHTPAIIEGDTLLITVVGGIILGFGMGLALRNGGALDGIDMLAVLLSRKLPFGTSDLILFLNLFVFIFVSTVFGLQGALLSVIAYYIASKVIHVVEEGLSGSKTFQIITTQ--PELMVETIRDQLGRSATYKEAYGGFSH----------------EKFKEITCVINRLEETKLKEIINDIDK-----TAFVTVYDVAEVKGSNFRNLNHH 4R25 Chain:A ((5-103)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KVEIVTRPANFEKLKQELGKIGVTSLTFSNVHGCGLQKAHTELYRGVKIESNVYERLKIEIVVSKVPVDQVTETAKRVLKTGSPGDGKIFVYEISNTIN---------

General information: TITO was launched using: RESULT: Template: 4R25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 262 -44787 -170.94 -589.30 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.46 3D Compatibility (PKB) : -170.94 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_4R25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R25-query.scw PDB file : Tito_Scwrl_4R25.pdb: