Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MEISDIIYGQHHI-DGVLEELIKSAPVQRLKGIYQGGASFLVNRKWNVTRYEHSIGVMLL-IKKLGGTIEE------------QIAGLLHDVSHTAFSHVVDVVFENQAEDYHENIFQ-Q-VI-VHS-EIPDILQKHGYHTEELLSDDSRWTLLEQPAPELCA-DRTDYTLRDMYRYGHINLH-EAQTFLD-HLIVRNGRMF-PDSIEAAEWFVSVYY---------------------------------------KEVIDFFLNPVNVYGYEYLARALKAALRHDVISAEDLLKT----DQEVLNILRAS-KNEEVLSLLTSIHPGIQVIEDDIQYDFHQKKKMRLIDPSIFLDDKWIKSS-GVSEK-----------VRKMGE------AAYQKAKKGVYIKILKQ 2Q14 Chain:A ((5-407)) ERKIINDPVFGFINIPKGLLYDIVRHPLLQRLTRIKQVGLSSVVYPGAQHTRFQHSLGAFYLMSEAITQLTSKGNFIFDSEAEAVQAAILLHDIGHGPFSHVLEDTIVQGVS--HEEISLMLMERMNKEMN------GQLSLAIQIFKDEYPKRFLHQLVSGQLDMDRLDYLRRDSFYTGVTEGNIGSARIIKMLDVADDRLVIESKGIYSIENFLTARRLMYWQVYLHKTSVAYERMLISTLLRAKELASQGVELFASPALHFFLYNDINHTEFHNNPDCLENFIQLDDNDIWTALKVWSNHPDKVLSTLSLGMINRNIFKVENSAEPIGEDRIKELTLQISQQLGITLSEANYFVSTPSIEKNMYDPADDSIDIIYKDGTIKNIAEASDMLNISLLSKKVKKYYLCYQR

General information: TITO was launched using: RESULT: Template: 2Q14.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1272 -113031 -88.86 -356.56 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -88.86 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.200

(partial model without unconserved sides chains): PDB file : Tito_2Q14.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q14-query.scw PDB file : Tito_Scwrl_2Q14.pdb: