Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFKVFLLAASTIAVGLVELIVGGILPQIAND----LDISIVSAGQLISVFALGYAVSGPLLLALTAKIERKRLYLIALFVFFLSNLVAYFSPN-------FATLMVSRVLAAMSTGLIVVLSLTIAPKIVAPEYRARAIGIIFMGFSSAIALGVPLGILISDS--------------FGWRILFLGIGLLALISMLIISIFFERIPAEKMIPFREQL-----KTIGN----LKIASSHLVTMFTLAGHYTLYAYFAPFLEETLHLSSFW----------VSICYFLFGISAVCGGPFGGALSDRLGSFKSILLVTGSFAIIMFLLPL-STS-S-MIFFLPVMVIWGLLSWSLAPAQQSYLIEIAPD-SSDIQQSFNTSALQVGIALGSAIGGVVLDQTGTVVSTAWCGGSIVIIAVLFAFISLTRPVQTAKKSSL 4J05 Chain:A ((40-496)) -------AGVGFFLDAYDLFIINQVAPMLAQVYFPKTGLPAQRQDLMKAAANIGCVVGQVMFGVLGDSFGRKFVYGKELILIIVATIFQMSAPSHWDGNRVLTWITICRVFLGIGIGGDYPMSATVVSDRANIHRRGTLLCFIFANQGWGSFVGSLVTIVTISGFKHRLKSGHTHDVDKAWRILIGLSLIPAFGTLYQ-R------------------WQEFVAYFSTWNHFRNLLGSMLGWFLVDIAFYGINLNQSVVLAQ-IGFAGKTGDVYDKLFQLATGNIIVTALGFLPGYYFTLFLIDIVGRKKLQFMGFIMSGLFLAILAGEIDHIGKGPLLACFTFMQFFFNFGANTTTFIVAAELFPTRIRASAHGISAAAGKCGAILSSLVFNQLKAKIG-TSAVLWIFFSTCILGFI------------------

General information: TITO was launched using: RESULT: Template: 4J05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1825 -297030 -162.76 -868.51 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -162.76 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_4J05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J05-query.scw PDB file : Tito_Scwrl_4J05.pdb: