Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMQTILSNGIAMVLIILIINIVYVSFFTIRMILTLKGQRYLAAGISTIEILVYVTGLSLVLDNLDQIQNVIAYALGYGLGVIVGMKIEEKLALGYIMVNVITKE-LDLDLPKQLREKG-YGVTNWVAGGLEG----DRTALQILTPRRYELQLYDTIKTLDS-----KAFIIAYEPKTIHGGFWVKAVKKRRIKE 2GW8 Chain:A ((3-101)) ------------------------------------------------------------------------------------------------KIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRVDFLPKIKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIR--------------

General information: TITO was launched using: RESULT: Template: 2GW8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 262 -38667 -147.58 -522.52 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.46 3D Compatibility (PKB) : -147.58 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_2GW8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GW8-query.scw PDB file : Tito_Scwrl_2GW8.pdb: