Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIEIIISLGIYFIAMLLIGWYAFKKTTDINDYMLGGRGLGPFVTALSAGAADMSGWMLMGVPGAMFATGLSTLWLALGLTIGAYSNYLLLAPRLRAYTEAADDAI------TIPDFFDKRFQHSSSLLKIVSALIIMIFFTLYTSSGMVSGGRLFESAFGADYKLGLFLTTAVVVLYTLFGGFLAVSLTDFVQGAIMFAALVLVPIVAFTHVGGVAPTFHEIDAVNPHLLDIFKGASV-ISIISYLAWGLGYYGQPHIIVRFMAIKDIKDLKPARRIGMSWMIITVLGSVLTGLIGVAYAHKFGVAVKDPEMIFIIF-SKILFHPLITGFLLSAILAAIMSSISSQLLVTASAVTEDLYRSFFRRKASDKELVMIGRLSVLVIAVIAVLLSL----NPNSTILDLVGYAWAGFGSAFGPAILLSLYWKRMNEWGALAAMIVGAATVLIWITTGLAKSTGVYEIIPGFILSMIAGIIVSMITKRPAKASYRLFGVMEKLLKRKK 4K7J Chain:A ((26-375)) ---------------------------GLLTDY----------------GNASASPWMKK-LQSVAQGSGETFRILQIGDSHTA-GDFFTDSLRKRLQKTWGDGGIGWVYPANVKGQRMAAVRHNGNWQSLTSR---------NNTGDFPLGGILAHTGSGGSMTLTASDGIASKQRVSLFAKPLLAEQTLTVNG-------------------------NTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFINIEN---------------PAGGITVSAMGIN--GAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID-----------IADTEQKWLDTVRQIRDSLPAA-----------------GILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVARQGQ-----TMFWS-------WQNAMGGICSMKNWLNQGWAAKDGVH-----------------FSAKGYRRAAEMLADSLEELVRSA-

General information: TITO was launched using: RESULT: Template: 4K7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1990 -238643 -119.92 -706.04 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -119.92 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_4K7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K7J-query.scw PDB file : Tito_Scwrl_4K7J.pdb: