Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKRITFGLLVLMVCVILFVLYVQLRNKDSIPVQSSAIHPEEDRIFFIYSNPFIKESVLLSTSTGERFNRRTFKVADVPYIQTKSYASTDLVLLAEHEPFYYTLEKDVIKEHPLSDPFAFWYEGKDVTIEAYNVDTTGNEIHINDRKTKKEYTLTLPPLVTMGASDENFIYIIQS----MSIYVIDRKTEEMIETLSLAS--YADQFADSEEFIVASS--DHKLTVIEKGTWKT-TYIAYPEDLEYADTVYYDKESGSFYVAYEDKEGGANLLEYGEDF-SI-HTYSLKFP------YMEAKFKGNLLYIVAQEEHKKGIGGYVGVFDIHSKEMLYQFDLPEEQVKVQDFVVVDNK 3MBR Chain:X ((22-190)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------FTEGLFYLRGHLYESTGETGRSSVRKVDLETGRILQRAEVPPPYFGAGIVAWRDRLIQLTWRNHEGFVYDLATLTPRARFRYP-----GEGWALTSDDSHLYMSDGT----AVIRKLDPDTLQQVGSIKVTAGGRPLDNLNELEWVNGELLANVW------LTSRIARIDPASGKVVAWIDLQA--------------

General information: TITO was launched using: RESULT: Template: 3MBR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 791 -91459 -115.62 -601.70 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain X : 0.59 3D Compatibility (PKB) : -115.62 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_3MBR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MBR-query.scw PDB file : Tito_Scwrl_3MBR.pdb: