Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METPETRFCKESKVVKTSRVFPLDTNNHNTLFGGKLMSYIDDIASISAARHCRRETVTASMDSVDFLKPIGQKDSVCLESYVTWVGTSSMEVFVKVIKEHLMTGERELAATSFLTFVALDSNGKPVPVPRVVPETEEEIMLHNTAVQRANERKNRKRHSQALANALGTDKPW 4IEN Chain:C ((14-153)) ------------ELIMSELMMPDTANFSGNVHGGELLLLLDQVAYSCASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTGEIRHTNSCYFTMVAV-KDGKPVPVPPL------EIL---TDRQRCRYEKAKKRRDISL----------

General information: TITO was launched using: RESULT: Template: 4IEN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 636 -63628 -100.04 -454.48 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -100.04 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_4IEN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IEN-query.scw PDB file : Tito_Scwrl_4IEN.pdb: