Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIYDAANWSKHEDDFTQMFYNQNVKQFWLPEEIALNGDLLTWKYLGKNEQDTYMKVLAGLTLLDTEQGNTGMPIVAEHVDGHQRKAVLNFMAMMENAVHAKSYSNIFLTLAPTEQINEVFEWVKNNRFLQKKARTIVSVYKTIKKNDEISLFKGMVASVFLESFLFYSGFYYPLYFYGQGKLMQSGEIINLIIRDEAIHGVYVGLLAQEIYKKQTPQKQKELYAWALNLLQELYENELEYTEDVYDQVGLAPDVKKFIRYNANKALNNLGFDHWFEE--EDVNPIVINGLNTKTKSHDFFSTKGNGYKKATVEPLKDSDFIFTEKGCIQ 4N83 Chain:F ((3-286)) --TYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDWRKLSHKEKDLVGKVFGGLTLLDTLQSESGVDALRKDVRTAHEEAVFNNIQFME-SVHAKSYSSIFSTLNTKSEIDEIFAWTNTNPYLQKKAEIINEIYLNG------TALEKKIASVFLETFLFYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLAFNELPEDEQEKLKEWMYDLLYTLYENEEGYTESLYDTVGWTEEVKTFLRYNANKALMNLGQDPLFPDSADDVNPIVMNGIS--------------------------------------

General information: TITO was launched using: RESULT: Template: 4N83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1290 -122761 -95.16 -435.32 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain F : 0.81 3D Compatibility (PKB) : -95.16 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_4N83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N83-query.scw PDB file : Tito_Scwrl_4N83.pdb: