Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MTPTKQHKKETFTHFIFRVFMILVGAASAAVSIELFLIPNDFIDGGIIGVSLILD-----HLFMSNPFLNFAFFVVILNIPFMIFGYKYIGKTF--------LVSTFI---GIVGLAVIESSLHHVEA---ITTQPILATVFGGLLLGFGVGLVIRNGGSMDGTEILGILLTKKLPFSVGEFVMFINVFIFIWAVFVFGPEQAMYSFMTYYIAMKTIDAVIQGLDETKAVIIVSEQY----DEISDAILHRLGRGTTKLKGKGGYTDEEKDVIYAVVTRLEVTKLKSIVFEVDQNAFITIMNTHETRGGKFKNAIH------- 2CEY Chain:A ((1-306)) ADYDLKFGMNAGTSSNEYKAAEMFAKEVKEKSQG-KIEISLYPSSQLGDDRAMLKQLKDGSLDFTFAESARFQLFYPEAAVFALPYVISNYNVAQKALFDTEFGKDLIKKMDKDLGVTLLSQAYNGTRQTTSNRAINSIADMK------------GLKLRVPNAATNLAYAKYVGASPTPMAFSEVYLALQ------TNAVDGQENPLAAVQAQKFYEVQKFLAMTNHILNDQLYLVSNETYKELPEDLQKVVKDAAENAAKYHTKLFVDGEKDLVTFFEKQGVKITHPDLVPFKESMKPYYAEFVKQT--GQKGESALKQIEAINP

General information: TITO was launched using: RESULT: Template: 2CEY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1432 -3621 -2.53 -13.31 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -2.53 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.186

(partial model without unconserved sides chains): PDB file : Tito_2CEY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CEY-query.scw PDB file : Tito_Scwrl_2CEY.pdb: