Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRIGSHVSMSGKHMLLAASQEAVSYGANTFMIYTGAPQNTRRKKIEDLNIEAGRAHMQE-NGIDEIIVHAPYIINIGNTTNPSTFELGVDFLRSEIERTAAIGAKQIVLHPGAHVGAGAEAGIKKIIEGLNEVIDPNQNVQIALETMAGKGSECGRSFEELAQIIEGVTHNEQLSVCFDTCHTHDAGYNIVEDFDGVLNEFDKIIGIDRIKVLHINDSKNVKGARKDRHENIGFGEIGFDALQYVVHHEQLK-DIPKILETPYVGEDKKNKKPPYRFEIEMLKEKQFDDTLLEKILQQ 3AAM Chain:A ((2-266)) -PRYGFHLSIAGKKGVAGAVEEATALGLTAFQIFAKSPRSWRPRALSPAEVEAFRALREASGGL-PAVIHASYLVNLGAE-G-ELWEKSVASLADDLEKAALLGVEYVVVHPGSGRP---ERVKEGALKALRLAGV-RSRPVLLVENTAGGGEKVGARFEELAWLVAD----TPLQVCLDTCHAYAAGYDVAEDPLGVLDALDRAVGLERVPVVHLNDSVGGLGSRVDHHAHLLQGKIG-EGLKRVFLDPRLKDR-VFILETPRGPE-------EDAWNLRVFRAW-------------

General information: TITO was launched using: RESULT: Template: 3AAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1505 -42790 -28.43 -162.70 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -28.43 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_3AAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3AAM-query.scw PDB file : Tito_Scwrl_3AAM.pdb: