Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MELIIILLALGLLMFTAYRGFSVILFAPICALFAVLLTDPSHVLPFFSSIFME---KMAGFIKLYFPVFLLGAIFGKVVEMAGLAASIAKTIVRLVGAKRAILAIVLMGAVLTYSGVSLFVVVFAVYPFAKNMFQEANI----PKRLIPGTIALGAFTFTMD---ALPGTPQIQNVIPTSFFKTDIYAAPWLGLMGAVI---VLAAGMLYLESRRK------KAQASGEGYGGFDSQNAPAPESIESAAEPDKSPIRHALAFVPLILVGAVNKYFTIYLPKWYPN----------GFDFSSIGLKEFGRLDISSAAAIWSVEIALVIGIITTILFDW--RSVFAQLKEGLNEGIGGALLASMNTGAEYG-FGGIIAALPGFHKLSSGISHTFTDPLVNGAVTTTALAGITGSASGG---------------MGIALSAMSEQYLQAIQAYNIPPEVMHRVISMASGGMDTLPHNGAVITLLAVTGLTHRQSYRDIFAITLIKTAAV----FAVIAIYSLTGLV------------------------------------------------------------------------------------------------------------------------------------------------------------------ 4UI9 Chain:O ((27-756)) KDWVTPYKIAVLVLLSEMSRTGEGAVSLVERRRLNQQLLPLLQGPDITVSKLLKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGT--EPEVHKTSVVGLFLRHMILAYSKLS-FSQVFKLYTALQQYFQSDENKKPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRSDSYVLLEHSVKKAVHFGLPYLASLGIQSLVQQRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEQQNNTESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGK--YHLADSLVTGITALNSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKEYRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPILADGAILDKGRAMFLVAKCQVASAASYDQPKKAEALEAAIENLNEAKNYFAKVDCKERIRDVVYFQARLYHTLGKTQERNRCAMLFRQLHQELPSVPLINHL

General information: TITO was launched using: RESULT: Template: 4UI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 1908 -88366 -46.31 -189.22 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain O : 0.61 3D Compatibility (PKB) : -46.31 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_4UI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UI9-query.scw PDB file : Tito_Scwrl_4UI9.pdb: