Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSKKKQIKLGVFLAGTGHHVASWRHPDAPSDASMNLDYFKELAKTAERGKLDMLFLADSLSIDSKSHPNVLTRFEPFTLLSALAQVTSKIGLTAT-ASTTYSEPFHIARQFASLDHLSNGRAGWNVVTSSIESTALNFSGEKH-LEHHLRYQRAEEFVEIVKGLWDSWEEDAFIRNKETGEFFDKEKMHELNHKGEYFSVRGPL---------NVSRTPQ-GQPVIIQAGSS--GDGKALAAKTAEVIFTAQNHLESAQEFYQSIKEQAAEFGRDPEKIAIMPGIFPIIADTEEAAQAKYKEL-QDLIIPSVGLQILQNYLGGIDLSAYPLDGPLPKLDAEASNAVKSRFKLVQEMAERDNMTIRELYKYVAGSRGHHIFVGTPEQLADKMQEWVDTK-ACDGFNIMPPL---L----PEGIEVFVDQVVPILQERGVFRKEYEGTTLREHFGLEKPVNRYAK 4UWM Chain:A ((2-376)) ------AMETGLIFHPY--MRP-----GR--SARQTFDWGIKSAVQADSVGIDSMMISEHASQIWE------NIPNPELLIAAAALQTKNIKFAPMAHLLPHQHPAKLATMIGWLSQILEGRYFLGIGAGAYPQASYMHGIRNAG--TKNLNDMVRESLFIMEKIWKR---------------------EPFFHEGKYWDAGYPEELEDEQHKLADFSPWGGKAPEIAVTGFSYNSPSMRLAGERNFKPVSIFSGLDALKRHWEVYSEAAIEAGHTPDRSRHAVSHTVFCADTDKEAKRLVMEGPIGYCFE-RYLIPIWRRFG------MMD-----------------GYAKDAGI-DPVDADLEFL-------VDNVFLVGSPDTVTEKINALFEATGGWGTLQVEAHDYYDDPAPWFQSLELISKEVAPKILLP---------------------------

General information: TITO was launched using: RESULT: Template: 4UWM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1734 -1531 -0.88 -4.53 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -0.88 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_4UWM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UWM-query.scw PDB file : Tito_Scwrl_4UWM.pdb: