Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVRFLQISDLHFQFQNY------DTIVMRDKLLDFIEVLRRESDFDFLLLTGDIAHKGDVYNEDVKEYLNGIIKSMGLSKNNVHLVPGNHDISRDMTRTLLIDSIMKSPNPSEMLDKLDQKATNILVEGQRKFFDFYEDFMGVKYPEEDLHFLYQSEKYNVLSINTCLLSDKKGEEGTLLTAQMKLYKAIRKMNKEKNKGKVLNIAIGHHTLGCIES--------SERESIKAHFDDY-FIDLYLAGHVHDSSFNITANTNENPFLELVSGAIIK-----------DEYATPEFISVDVNLDNGETEVTYYIWNTEYKYWSKNNQGGRRLQEGKLNYKINRLESLIEKEIEDNDDKIDEDEFKSFIIDFHEYRESYKTFTSNFDNQIGLDKKFYDMKSGETFKRKFDSYSEYFGVINHIMDSTSYVSADKKELIAETIVDKYLEFHNQYNNGDEIFVKIVNEIYLECHSVLPYSKALTKKYIKILTCWCIYECEIFNDNKRSVKND 3D03 Chain:A ((1-262)) --MLLAHISDTHFRSRGEKLYGFIDVNAANADVVSQLNAL--RERPDAVVVSGDIVNCGRPEE---YQVARQILG---SLNYPLYLIPGNHDDKALFLEYL-------------------Q----PLC----------PQLGSD---ANNMRCAVDDFATRLLFIDSSRAGTS---KG--WLTDETISWLEAQLFEGGD---KPATIFMHHPPLPLGNAQMDPIACENGHRLLALVERFPSLTRIFCGHNHSLTMTQ-YR---QALISTLPGTVHQVPYCHADTDPYYDLSPASCLMHRQVGE--QWVSYQHSLAHYAGPWL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3D03.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1327 24657 18.58 104.48 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 18.58 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_3D03.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D03-query.scw PDB file : Tito_Scwrl_3D03.pdb: