Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MFNTAVKILY--RSLIELTNHRLSSYLIKGFCESKISKPVIPLFSKHFRLNWDDVDGTAADYGSLSELFIRQINLERRPVSKEAHAVVSPVDGVVQTVGIINPNQTFTVKGKDYSFAELTGCKSADHQYNG--GYFVVLYLSPRHYHRFHSPISCRYQKLAELGNRSYPVNQLGLK-YGKDVLSKNYRFVYELNSGSRNVLMIPVGAMNINSIVQTNTRTELEIGEELGYFSFGSTVILVFEKDAFQPSAHLAEGQEVQVGELIG--YEE 1FUI Chain:A ((356-591)) AQVFADVRTYWSPEAIERVTGHKLDGLAEHG---------IIHLINS----GSAALDGSCKQRDSEGN---PTMKPHWEISQQEADACLAA------TEWCPAIHEYFRGGGYSSRFLTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDV---HDILNKRTNSTWPTTWFAPRLTGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETK----------VYRPSAWAAHGMDIEGQDYRACQNYGPLYKR

General information: TITO was launched using: RESULT: Template: 1FUI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1091 -59946 -54.95 -262.92 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -54.95 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.178

(partial model without unconserved sides chains): PDB file : Tito_1FUI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FUI-query.scw PDB file : Tito_Scwrl_1FUI.pdb: