Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATIDLQKKSVKIVLEKKQLTKVTARVGLVLDITGSMRPLYKNGTVQNVVERILAVADQFDDNGLLDVWVYDNEFSRLKPVS-----E--KDFSGYVDREILNNDRLHKFGRNDEPPVMKDVLRKYVTEEPSSYPAFIVFINDGGCKK----SIKPIIEASSDKPVFWQFVGIGN-GNFDFLNKLDTLEGRVIDNTNFLHIEEIDRISDDELYDALLAEFPFWLKEAKEKGIVREQEPPAEKPKKKGFFSRLFSK 4FX5 Chain:A ((76-246)) ----------------------SENVEVIIIDCSGSMDYPR--TKMMAAKEATKVAIDTLTDGAFFAVVAGTEGARVVYPTGGQLLRADYQSRAA-AKEAVGR---LHANGGTAMGRWLAQAGRIFDT--APSAIKHAILLTDGKDESETPADLARAIQS-SIGNFTADCRGIGEDWEPKELRKIADAL-----LGTVGIIRDPATL------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FX5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 831 -87102 -104.82 -547.81 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -104.82 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_4FX5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FX5-query.scw PDB file : Tito_Scwrl_4FX5.pdb: