Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQYASES-------INLPGEITFKDVREIFFYQIAKISCFYFLLFCAIFAAVNFINGWPRIVYGSDALNLFMNSMLIIVMSVLFTLLLLLLLYVKFSRAYKKNERMKSKRTYTLNQEGIRICSKKYDLIFNWDEITAVFEYKN----IFRVNTSSGQYIAIPKHFFHSEEEMNRFKEIILKNTETKKLKFKKDQH 4OWK Chain:A ((4-134)) MAHVTLQSLSNNDLCLDVYGENGDKTVAGG---SVNGWSC----------------HGSWNQVWGLDKEE-------------------------RYRSRVASDRCLTVNADKTLTVEQ---CGANLAQKWYWEGDKLISRYVDGNNTRYLLNIVGGR------------------NVQVTPENEANQARWKPTLQ

General information: TITO was launched using: RESULT: Template: 4OWK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 597 -15990 -26.78 -133.25 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -26.78 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.116

(partial model without unconserved sides chains): PDB file : Tito_4OWK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OWK-query.scw PDB file : Tito_Scwrl_4OWK.pdb: