Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIHIVGPGDSLFSIGRRYGASVDQIRGVNGLDETNIVPGQALLIPLYVYTVQPRDTLTAIAAKAFVPLERLRAANPGISPNALQAGAKITIPSISNYIAGTLSFYVLRNPDLDRELINDYAPYSSSISIFEYHIAPNG-DIANQLNDAAAIETTWQRRVTPLATITNLTS--GGFSTEIVHQVLNNPTARTNLVNNIYDLVSTRGYGGVTIDFEQVSAADRDLFTGFLRQLRDRLQAGGYVLTIAVPAKTSD-NIPWLRGYDYGGIGAVVNYMFIM-AYDWH---------HAGSEPGPVAPITEIRRTIEFTIAQVPSRKIIIGVPLYGYDWIIPYQPGTVASAISNQNAIERAMRYQAPIQYSAEYQSPFFRYSD-----------QQGRTHEVWFEDVRSMSRKMQIVREYRLQAIGAWQL-TLGFTPGPWLLRKFFTIRKV 4Q6T Chain:A ((4-340)) --------------------------------------------------------------------------------------------APFVLAYTDGQVEASYSNLQAFHRNLSAVGLGSTYGLTVTGKLRQDGMN----ETTQNIIRFAKSQSLPLYPTVSDYNEDIGAFDPAISHSILNDRALSAGTVKQLVKLAKEGGFAGINLDFEKVEPRNRAAFCAFVKTLGNALHASNKKLIISIPPKLSDTEPEYLQGYDYKALGAAVDYFQ-VMTYDQVGPGWSSGGFHNEAWPGPESGFDWQQALLSYAVSRVPASKVLAGLPTYGQDYSIG-------NRVHWSAYQEIIAEHRAAIHRDAASATPYATWGPVKSFVDGVEWTPERAQPVLWYDDAASIKTKTALVTRLGLGGTSVW-AMGYENAGFWAALQSGLK----

General information: TITO was launched using: RESULT: Template: 4Q6T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1804 -174720 -96.85 -561.80 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -96.85 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_4Q6T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q6T-query.scw PDB file : Tito_Scwrl_4Q6T.pdb: