Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVVSLFSGIGGIELGLHQSG------HTTEIFCEVDPLAKAVLSKNFPGVK------IEDD-----------------------------------------INEIR--ELP-SCDLVAAGFPCQDLSQAGGKEGID---GSRSGLVKKLFELIEKKE----HANRPPWILIENVPYMLRLNRGKAMSYLTSVLSELGYTWAYRTVDARCFGLPQRRHRVILLASLFEDPK--DVIFSQDH----SEPDLDGKPSVVDHSNYY------GFYW---------TE---------GLRGVGWAREAVPPIKCGSSVGIASPPAVWSPYEDIVGTINIRDAERLQGFP-EDWTNITTETGK--DIKEGARWRLVGNAVSVRVSKWIGENLSQPKGSISDFEGELVTKTWPSAAWGYGDKKYKVPVSKWVANTEQIAISEFLNHPLKPLSARALNGFLGRAARCTNVNYSDEFINSLERCKDRQLQKV 4DKJ Chain:A ((11-393)) IKVFEAFAGIGSQFKALKNIARSKNWEIQHSGMVEWFVDAIVSYVAIHSKNFNPKIERLDRDILSISNDSKMPISEYGIKKINNTIKASYLNYAKKHFNNLFDIKKVNKDNFPKNIDIFTYSFPCQDLSVQGLQKGIDKELNTRSGLLWEIERILEEIKNSFSKEEMPKYLLMENVKNLLSHKNKKNYNTWLKQLEKFGYKSKTYLLNSKNFDNCQNRERVFCLSIRDDYLEKTGFKFKELEKVKNPPKKIKDILVDSSNYKYLNLNKYETTTFRETKSNIISRPLKNYTTFNSENYVYNINGI-GPTLTASGAN---SR--IKIETQQGVRYLTPLECFKYMQFDVNDFK----KVQSTNLISENKMIYIAGNSIPVKILEAIFNTLEFVNN--------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DKJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1538 -124423 -80.90 -433.53 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -80.90 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_4DKJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DKJ-query.scw PDB file : Tito_Scwrl_4DKJ.pdb: