Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQYEELNKQFIGGKWQEGSSPNVLENKNPYTQKTFTTFRKATADDVDEAYRAAALAKKKWDAVNPFEKRTILEKAVTYIEENEEAIIYLIMEELGGTRLKAAFEIGLVKNIIKEAATFPIRMEGKILPSTIDGKENRLYRVPAGVVGVISPFNFPFFLSMKSVAPALGAGNGVVLKPHEETPICGGTLIAKIFENAGIPAGLLNVVVTDIAEIGDSFVEHPVPRIISFTGSTKVGSYIGQLAMKHFKKPLLELGGNSAFIVLEDADIEYAVNAAVFSRFTHQGQICMSANRVLVHSSIYDKFLELYQAKVESLKVGDPMDPDTI-IGPLINSRQTDGLMKTVEQAIEEGAVPVKLGGFNGTIVEPTILKDVKPFMSIAKEELFGPVVSFMKFDSEDEAVDIANETPFGLSGAVHTSNLERGVAFAKRIETGMIHVNDTTINDEPNVAFGGEKQSGLGRLNGEWSLEEFTTLKWISVQHEKRSFPY 4NMK Chain:B ((11-485)) ---------NYINGEFKEPSTGAFQVKTSPVDGSKIAEVPRSGREDAREAIDSAFEALKAWANIPAIRRAEYLYKMLEVFRQMKEDFMKILTVEGGGTYRKVWGEVVFTERLIQNAAELARHYQGRVLQSDSESTISVVFKRSKGVVGVITPWNYPLSISMKKIAHTLAVGNTVVYKPASDTPVTG-WLIAQMVAKAGLPKGVFNLVIGPGPVVGEEIVTHKRVAHVTFTGESSTGREIAAKAAGTLKTVTLELGGSDPLIILDDVDVDYAARLAVFASLFHQGQICTSAKRIIVHKAVADKFIERYVHYVKMLRIDDPRKDEKVDLGPLINERQVALMKEFVDDAVSRGGRLLIGGRSWGNFFEPAIFVDVDRNFRIMREEVFGPVRPIVVVENDDQAVEVANDTDYGLSGAVLTNNVNRAFRIAEAVESGMFHINDVTFLEESHVPFGGIKASGVGREGGEWSFHETTYDRWVTVTLRTRRFP-

General information: TITO was launched using: RESULT: Template: 4NMK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2927 -206805 -70.65 -436.30 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -70.65 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_4NMK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NMK-query.scw PDB file : Tito_Scwrl_4NMK.pdb: