Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEALTEQKSQKWAISLFTIGVFMAALDNGIISAALTTINESF--------SVSPSWGSWGITLYTLGLSVSVPIVGKLSDRYGRKKLFLIEVCLFGLGSLLVAL------------------SQSFPLFLISRLIQALGGGGIFIIGSSHILATLPKEKQGKALGLLGAMNGMAAVLGPNIGSFLLDW-------TGSWHWLFLINLPIAVLLVVFGACFIAETKAPEAKRLDAAGIFLLSLSILAVMYGMTNLDGANLLHSLGNPEVYGCIIFGILCFAALISYEKRVEMRGGDPILAYSLLRN--HMFQRTLIIGLL-SGGLL-AAVIFIPSYVEQYLGVPAAKAGYWMTPLALASGIGAWLGGALTDKKGPVKTVILSGIISCAGFALFPLWV--T-EKWEFVIASVAAGIGFGFLLGAPLNVLVSEAAK-TNKGTALGTLSLVRQIGLTLAPTLYAGFITAGFDQIGDEINSRLSDSGYSEKAMQMIPEIDSSEVSSLQEQIERIPVPEVKTAISDAIHASVASGYDHLYAAAAVVSLLVIAAISIPAFRRQKR 4GC0 Chain:A ((11-429)) -----------FSITLVATLGGLLFGYDTAVISGTVESLNTVFVAPQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLML-LYTVPESPRWLMSRGKQEQAEGIL----------RKIMG--------NTLAT-------------QAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFK-TLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMM------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4GC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1937 -256382 -132.36 -678.26 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -132.36 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_4GC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GC0-query.scw PDB file : Tito_Scwrl_4GC0.pdb: