Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNQFQSSKPIYLQIADQIFYRLVRKELLPGDKLPSVREMAIQTKVNPNTIQRTYSEMERLGIVETRRGQGTFIAEKAEIVDELKDKLTREV--LEGFVKQMKELGLTKEEMLEGIKTFTEGG 2WV0 Chain:D ((1-99)) MNINKQSPIPIYYQIMEQLKTQIKNGELQPDMPLPSEREYAEQFGISRMTVRQALSNLVNEGLLYRLKGRGTFVS---------KPKMEQALQGLTSFTEDMKSRGMT---------------

General information: TITO was launched using: RESULT: Template: 2WV0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 326 -23118 -70.91 -238.32 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : -70.91 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.669

(partial model without unconserved sides chains): PDB file : Tito_2WV0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WV0-query.scw PDB file : Tito_Scwrl_2WV0.pdb: