Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSMDHRIERLEYYIQLLVKTVDMDRYPFYALLIDKGLSKEEGEAVMRICDELSEELATQKAQGFVTFDKLLALFAGQLNEKLDVHETIFALYEQGLYQELMEVFIDIMKHFD 1G8E Chain:A ((1-98)) MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMATTLAALTLPQMVKLAETNQL--------------VCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLST-

General information: TITO was launched using: RESULT: Template: 1G8E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 191 -25326 -132.60 -258.43 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -132.60 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_1G8E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G8E-query.scw PDB file : Tito_Scwrl_1G8E.pdb: